The Wisconsin Department of Natural Resources has identified a new State Natural Area in the Lower Chippewa River region of western Wisconsin. This 100,000-ha area contains 800 ha of prairie remnants—25% of Wisconsin's remaining remnant prairie. Diversity of grasshopper populations (Orthoptera: Acrididae) was assessed in these remnant prairies. Remnants ranged in size from <1 ha to > 60 ha, and contained from 6 to 15 species from four subfamilies. Half of the remnants contained 10 or more species. Data from this survey provides the Wisconsin Department of Natural Resources with a tool to evaluate remnant prairies, and a snapshot of what grasshopper communities may have looked like in Wisconsin prior to settlement. This research also updates the Orthopteran literature database for acridid distribution records for this region of the USA.